VALIDATION OF REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION FOR INDIRECT ASSESSMENT OF FMD VIRUS TITRE
نویسندگان
چکیده
منابع مشابه
Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran
Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...
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Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome generates robust data that are informative and, most importantly, generally applicable. This is because the information contained within the genome is context-independent; i.e., generally, every normal cell contains the same DNA sequence--the same mutations and polymo...
متن کاملDevelopment and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses
Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans. Highly pathogenic avian influenza viruses (HPAI) H5N1 were first identified in Southeast Asia in 1996 and spread to four continents over the following years. The viruses have caused high mortality in chickens and various bird species ...
متن کاملdiagnosis of foot-and-mouth disease virus by real time reverse transcription polymerase chain reaction assay in iran
background and aims: accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (fmd). in present study, was evaluated real time reverse transcription-polymerase chain reaction (rrt-pcr) assay along with diagnostic routine methods for the detection of all seven serotypes of fmd virus (fmdv), namely o, c, a, sat1, 2, 3 and asia 1 in biological sample...
متن کاملDevelopment, optimization, and validation of a Classical swine fever virus real-time reverse transcription polymerase chain reaction assay.
Classical swine fever (CSF) is an economically devastating disease of pigs. Instrumental to the control of CSF is a well-characterized assay that can deliver a rapid, accurate diagnosis prior to the onset of clinical signs. A real-time fluorogenic-probe hydrolysis (TaqMan) reverse transcription polymerase chain reaction (RT-PCR) for CSF was developed by the United States Department of Agricultu...
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ژورنال
عنوان ژورنال: Veterinary Science Today
سال: 2019
ISSN: 2658-6959,2304-196X
DOI: 10.29326/2304-196x-2019-1-28-43-50